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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GARS Monoclonal antibody (67893-1-Ig)

    WB analysis using 67893-1-Ig (same clone as 67893-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67893-1-Ig (GARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GARS Monoclonal antibody (67893-1-Ig)

    WB analysis of HepG2 using 67893-1-Ig (same clone as 67893-1-PBS)

    WB result of GARS antibody (67893-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARS transfected HepG2 cells. This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GARS Monoclonal antibody (67893-1-Ig)

    IHC staining of human skin cancer using 67893-1-Ig (same clone as 67893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67893-1-Ig (GARS antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GARS Monoclonal antibody (67893-1-Ig)

    IHC staining of human skin cancer using 67893-1-Ig (same clone as 67893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67893-1-Ig (GARS antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GARS Monoclonal antibody (67893-1-Ig)

    IF Staining of A431 using 67893-1-Ig (same clone as 67893-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GARS antibody (67893-1-Ig, Clone: 2H9D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GARS Monoclonal antibody, PBS Only

GARS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67893-1-PBS
Clone No.2H9D3

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AP 4 A synthetase, CMT2D, DSMAV, GARS, Glycine tRNA ligase, glycyl tRNA synthetase, GlyRS, HMN5, SMAD1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67893-1-PBS targets GARS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GARS fusion protein Ag8432 相同性解析による交差性が予測される生物種
Full Name glycyl-tRNA synthetase
Calculated molecular weight 739 aa, 83 kDa
Observed molecular weight75-80 kDa
GenBank accession numberBC007755
Gene Symbol GARS
Gene ID (NCBI) 2617
RRIDAB_2918649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41250
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Glycyl-tRNA synthetase 1 (GARS, GeneID:2617) is one of the aminoacyl-tRNA synthetases. It is essential for protein synthesis in all cells and tissues (PMID: 17035524) and charges tRNA molecules with glycine by catalyzing the ATP-dependent ligation of glycine to the 3'-end of it's cognate tRNA via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PMID: 17544401, PMID: 28675565, PMID: 24898252) . GARS is located in cytoplasm, but also can be secreted from cells and as an extracellular protein which may influence cell signaling. Mutation of GARS causes peripheral neuropathies such as CMT2D, SMA (PMID: 26503042, PMID: 32181591) , or associates with cancer. Two transcript variants encoding different isoforms have been found for this gene. The molecular weight of isoform2 is about 78 kDa.