Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HepG2 cells, HeLa cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13659-1-AP targets GART in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GART fusion protein Ag4555 相同性解析による交差性が予測される生物種 |
| Full Name | phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase |
| Calculated molecular weight | 1010 aa, 108 kDa |
| Observed molecular weight | 108 kDa, 46 kDa |
| GenBank accession number | BC038958 |
| Gene Symbol | GART |
| Gene ID (NCBI) | 2618 |
| RRID | AB_2107937 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22102 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GART is a trifunctional polypeptide which has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity that is required for de novo purine biosynthesis. GART has 2 isoforms with molecular mass of 108 kDa and 46 kDa due to alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GART antibody 13659-1-AP | Download protocol |
| IHC protocol for GART antibody 13659-1-AP | Download protocol |
| IF protocol for GART antibody 13659-1-AP | Download protocol |
| IP protocol for GART antibody 13659-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle. | ||
Mol Cell ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis. | ||
Nat Commun Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway. | ||
EMBO J UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism. | ||
Cell Rep Purine Nucleotide Availability Regulates mTORC1 Activity through the Rheb GTPase. | ||