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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GART Monoclonal antibody

GART Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A11E2

Cat no : 67939-1-Ig

Synonyms

AIR synthase, AIRS, GAR transformylase, GARS, GART, GARTF, PAIS, PGFT, PRGS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GART Monoclonal antibody (67939-1-Ig)

    WB analysis using 67939-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GART Monoclonal antibody (67939-1-Ig)

    WB analysis using 67939-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GART Monoclonal antibody (67939-1-Ig)

    IF Staining of HeLa using 67939-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GART antibody (67939-1-Ig, Clone: 2A11E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67939-1-Ig targets GART in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GART fusion protein Ag30509 相同性解析による交差性が予測される生物種
Full Name phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
Calculated molecular weight 1010 aa, 108 kDa
Observed molecular weight 46 kDa, 108 kDa
GenBank accession numberBC038958
Gene symbol GART
Gene ID (NCBI) 2618
RRIDAB_2918691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GART antibody 67939-1-IgDownload protocol
IF protocol for GART antibody 67939-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Hua (Verified Customer) (11-11-2022)

Great antibody. Very strong signal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:8000
  • Cell Tissue Type: HepG2 cell/ Mouse liver
GART Antibody Western Blot validation (1:8000 dilution) in HepG2 cell/ Mouse liver (Cat no:67939-1-Ig)