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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GAS1 Monoclonal antibody

GAS1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H8E1

Cat no : 67181-1-Ig

Synonyms

GAS 1, GAS1, growth arrest 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAS1 Monoclonal antibody (67181-1-Ig)

    WB analysis using 67181-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67181-1-Ig (GAS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using GAS1 Monoclonal antibody (67181-1-Ig)

    WB analysis of SH-SY5Y using 67181-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67181-1-Ig (GAS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using GAS1 Monoclonal antibody (67181-1-Ig)

    WB analysis of pig brain using 67181-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67181-1-Ig (GAS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GAS1 Monoclonal antibody (67181-1-Ig)

    IHC staining of human gliomas using 67181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67181-1-Ig (GAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GAS1 Monoclonal antibody (67181-1-Ig)

    IHC staining of human gliomas using 67181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67181-1-Ig (GAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, SH-SY5Y cells, pig brain tissue, Y79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67181-1-Ig targets GAS1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Reactivity Human, Mouse, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GAS1 fusion protein Ag12259 相同性解析による交差性が予測される生物種
Full Name growth arrest-specific 1
Calculated molecular weight 345 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC074908
Gene symbol GAS1
Gene ID (NCBI) 2619
RRIDAB_2882477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GAS1 is involved in growth suppression. It is an integral plasma membrane protein whose expression is linked to growth arrest. It can be used as a novel therapeutic candidate for gastric cancer.

Protocols

Product Specific Protocols
WB protocol for GAS1 antibody 67181-1-IgDownload protocol
IHC protocol for GAS1 antibody 67181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols