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KD/KO Validated

GATA1 Polyclonal antibody

Name: GATA1 Polyclonal antibody
Brand: Proteintech
SKU: 10917-2-AP

GATA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10917-2-AP

Synonyms

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT

Validation Data Gallery

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AllWBIPIHC

WB analysis using 10917-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10917-2-AP (GATA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10917-2-AP
WB result of GATA1 antibody (10917-2-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA1 transfected K-562 cells.
WB analysis of Raji using 10917-2-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10917-2-AP (GATA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 10917-2-AP
IP result of anti-GATA1 (IP:10917-2-AP, 4ug; Detection:10917-2-AP 1:500) with K-562 cells lysate 4000ug.
IHC staining of mouse testis using 10917-2-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10917-2-AP (GATA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10917-2-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10917-2-AP (GATA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 10917-2-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HL-60 cells, Raji cells, K-562 cells, NIH/3T3 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 8 publications below
IHC	See 5 publications below
IF	See 1 publications below
ChIP	See 1 publications below

Product Information

10917-2-AP targets GATA1 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GATA1 fusion protein Ag1350 相同性解析による交差性が予測される生物種
Full Name	GATA binding protein 1 (globin transcription factor 1)
Calculated molecular weight	43 kDa
Observed molecular weight	50-55 kDa, 40-45 kDa
GenBank accession number	BC009797
Gene symbol	GATA1
Gene ID (NCBI)	2623
RRID	AB_2108279
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757]. GATA1 exists two isoform with the molecular weight 50-55 kDa and 40-45 kDa (PMID: 31189107 ).

Protocols

Product Specific Protocols
WB protocol for GATA1 antibody 10917-2-AP	Download protocol
IHC protocol for GATA1 antibody 10917-2-AP	Download protocol
IP protocol for GATA1 antibody 10917-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,ChIP	Theranostics Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p Authors - Soo Hyun Kang KD Validated View Article
human	WB,IHC	Oncogene microRNA-23a, -27a and -24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia. Authors - R Su View Article
human	WB	Front Immunol FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome. Authors - Chunlin Wang View Article
mouse	IHC	Genes (Basel) Proof of Gene Doping in a Mouse Model with a Human Erythropoietin Gene Transferred Using an Adenoviral Vector. Authors - Takehito Sugasawa View Article
human	WB,IHC	Oncotarget The transcription factor GATA1 and the histone methyltransferase SET7 interact to promote VEGF-mediated angiogenesis and tumor growth and predict clinical outcome of breast cancer. Authors - Yanan Zhang View Article
human	WB	Eur J Pharmacol Alnustone promotes megakaryocyte differentiation and platelet production via the interleukin-17A/interleukin-17A receptor/Src/RAC1/MEK/ERK signaling pathway Authors - Yueyue Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GATA1 Polyclonal antibody

GATA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 10917-2-AP

WB analysis of K-562 using 10917-2-AP

WB analysis of Raji using 10917-2-AP

IP experiment of K-562 using 10917-2-AP

IHC staining of mouse testis using 10917-2-AP

IHC staining of mouse testis using 10917-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p

microRNA-23a, -27a and -24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia.

FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.

Proof of Gene Doping in a Mouse Model with a Human Erythropoietin Gene Transferred Using an Adenoviral Vector.

The transcription factor GATA1 and the histone methyltransferase SET7 interact to promote VEGF-mediated angiogenesis and tumor growth and predict clinical outcome of breast cancer.

Alnustone promotes megakaryocyte differentiation and platelet production via the interleukin-17A/interleukin-17A receptor/Src/RAC1/MEK/ERK signaling pathway