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  • Featured Product
  • KD/KO Validated

GATA1 Monoclonal antibody, PBS Only

GATA1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5E2A8

Cat no : 60011-1-PBS

Synonyms

GF-1, GF1, GATA-binding factor 1, GATA-1, ERYF1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GATA1 Monoclonal antibody (60011-1-Ig)

    WB analysis using 60011-1-Ig (same clone as 60011-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using GATA1 Monoclonal antibody (60011-1-Ig)

    WB analysis of Raji using 60011-1-Ig (same clone as 60011-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using GATA1 Monoclonal antibody (60011-1-Ig)

    IHC staining of human breast hyperplasia using 60011-1-Ig (same clone as 60011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using GATA1 Monoclonal antibody (60011-1-Ig)

    IHC staining of human breast hyperplasia using 60011-1-Ig (same clone as 60011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using GATA1 Monoclonal antibody (60011-1-Ig)

    IHC staining of human breast hyperplasia using 60011-1-Ig (same clone as 60011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using GATA1 Monoclonal antibody (60011-1-Ig)

    IHC staining of human breast hyperplasia using 60011-1-Ig (same clone as 60011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60011-1-PBS targets GATA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATA1 fusion protein Ag1350 相同性解析による交差性が予測される生物種
Full Name GATA binding protein 1 (globin transcription factor 1)
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC009797
Gene symbol GATA1
Gene ID (NCBI) 2623
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].