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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GATA3 Polyclonal antibody, PBS Only

GATA3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22343-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using GATA3 Polyclonal antibody (22343-1-AP)

    WB analysis of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using GATA3 Polyclonal antibody (22343-1-AP)

    WB analysis of mouse thymus using 22343-1-AP (same clone as 22343-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GATA3 Polyclonal antibody (22343-1-AP)

    WB analysis using 22343-1-AP (same clone as 22343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GATA3 Polyclonal antibody (22343-1-AP)

    IF Staining of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (22343-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22343-1-PBS targets GATA3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GATA3 fusion protein Ag17833 相同性解析による交差性が予測される生物種
Full Name GATA binding protein 3
Calculated molecular weight 443 aa, 48 kDa
Observed molecular weight 37 kDa and 48 kDa
GenBank accession numberBC006793
Gene symbol GATA3
Gene ID (NCBI) 2625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Trans-acting T-cell-specific transcription factor, also known as GATA-3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin and mammary gland (PMID: 25148023). GATA-3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full length GATA3 protein contains 444 aa corresponding to the molecular weights of 48,0 kDa, and the truncated protein was deteced at approximately 37 kDa (PMID: 24758297).