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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GATA3 Recombinant antibody, PBS Only

GATA3 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240684A2

Cat no : 83808-1-PBS

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684A2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GATA3 Recombinant antibody (83808-1-RR)

    WB analysis using 83808-1-RR (same clone as 83808-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using GATA3 Recombinant antibody (83808-1-RR)

    WB analysis of MCF-7 using 83808-1-RR (same clone as 83808-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using GATA3 Recombinant antibody (83808-1-RR)

    FC experiment of Jurkat using 83808-1-RR (same clone as 83808-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U937 using GATA3 Recombinant antibody (83808-1-RR)

    FC experiment of U937 using 83808-1-RR (same clone as 83808-1-PBS)

    1x10^6 U937 were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83808-1-RR

    Affinity and Kinetic Characterization of 83808-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83808-1-RR against Human GATA3 were performed. The affinity constant is 0.686 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83808-1-PBS is GATA3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GATA3 fusion protein Ag0174 相同性解析による交差性が予測される生物種
Full Name GATA binding protein 3
Calculated molecular weight 443 aa, 48 kDa
Observed molecular weight 37 kDa and 48 kDa
GenBank accession numberBC003070
Gene symbol GATA3
Gene ID (NCBI) 2625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Trans-acting T-cell-specific transcription factor, also known as GATA3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin, and mammary gland (PMID: 25148023). GATA3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full-length GATA3 protein contains 444 aa corresponding to the molecular weights of 48 kDa, and the truncated protein was detected at approximately 37 kDa (PMID: 24758297).