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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GATA3 Recombinant antibody (83808-2-RR)

    IF Staining of MCF-7 using 83808-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (83808-2-RR, Clone: 240684B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of Jurkat cells using GATA3 Recombinant antibody (83808-2-RR)

    FC experiment of Jurkat using 83808-2-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-2-RR, Clone:240684B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of U937 using GATA3 Recombinant antibody (83808-2-RR)

    FC experiment of U937 using 83808-2-RR

    1x10^6 U937 were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-2-RR, Clone:240684B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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GATA3 Recombinant antibody

GATA3 Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83808-2-RR
Clone No.240684B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684B8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inJurkat cells, U-937 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83808-2-RR targets GATA3 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GATA3 fusion protein Ag0174 相同性解析による交差性が予測される生物種
Full Name GATA binding protein 3
Calculated molecular weight 443 aa, 48 kDa
GenBank accession numberBC003070
Gene Symbol GATA3
Gene ID (NCBI) 2625
RRIDAB_3671396
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This is a rabbit polyclonal antibody raised against part of C-terminus of human GATA3.

Protocols

Product Specific Protocols
IF protocol for GATA3 antibody 83808-2-RRDownload protocol
FC protocol for GATA3 antibody 83808-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols