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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GATA4 Monoclonal antibody

GATA4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F5C6

Cat no : 68012-1-Ig

Synonyms

GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using GATA4 Monoclonal antibody (68012-1-Ig)

    WB analysis of PC-3 using 68012-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GATA4 Monoclonal antibody (68012-1-Ig)

    WB analysis of HEK-293 using 68012-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GATA4 Monoclonal antibody (68012-1-Ig)

    WB analysis of HepG2 using 68012-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using GATA4 Monoclonal antibody (68012-1-Ig)

    WB analysis of NCCIT using 68012-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, HepG2 cells, NCCIT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68012-1-Ig targets GATA4 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATA4 fusion protein Ag30392 相同性解析による交差性が予測される生物種
Full Name GATA binding protein 4
Calculated molecular weight 442 aa, 45 kDa
Observed molecular weight44,45 kDa
GenBank accession numberBC101580
Gene symbol GATA4
Gene ID (NCBI) 2626
RRIDAB_2918759
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GATA4 is a transcriptional activator. GATA4 binds to the consensus sequence 5'-AGATAG-3'. GATA4 acts as a transcriptional activator of ANF in cooperation with NKX2-5. Mutation of GATA4 will cause the atrial septal defect type 2 (ASD2).

Protocols

Product Specific Protocols
WB protocol for GATA4 antibody 68012-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols