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GATA6 Polyclonal antibody

Name: GATA6 Polyclonal antibody
Brand: Proteintech
SKU: 55435-1-AP

GATA6 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 55435-1-AP

Synonyms

Transcription factor GATA-6, Transcription factor GATA 6, GATA-binding factor 6

Validation Data Gallery

Filter:

AllWBIHCFC (Intra)

WB analysis of MCF-7 using 55435-1-AP
WB result of GATA6 antibody (55435-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA6 transfected MCF-7 cells.
WB analysis using 55435-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55435-1-AP (GATA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 55435-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55435-1-AP (GATA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 55435-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 55435-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 55435-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 55435-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HuH-7 using 55435-1-AP
1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human GATA6 (55435-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 55435-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, BxPC-3 cells, MCF-7 cells
Positive IHC detected in	human colon cancer tissue, rat liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HuH-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IF	See 1 publications below

Product Information

55435-1-AP targets GATA6 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	GATA binding protein 6
Calculated molecular weight	60 kDa
Observed molecular weight	60-64 kDa
GenBank accession number	NM_005257
Gene symbol	GATA6
Gene ID (NCBI)	2627
RRID	AB_11182815
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GATA6 is thought to be important for regulating terminal differentiation and/or proliferation.

Protocols

Product Specific Protocols
WB protocol for GATA6 antibody 55435-1-AP	Download protocol
IHC protocol for GATA6 antibody 55435-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Environ Sci Technol Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling. Authors - Zhihua Ren View Article
mouse	WB,IF	iScience Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency Authors - Qian Ban View Article
human	WB	FASEB J Pulmonary expression of CYP2A13 and ABCB1 is regulated by FOXA2 and their genetic interaction is associated with lung cancer. Authors - Chan Xiang View Article
mouse	WB	Front Cell Dev Biol ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4. Authors - XiaoYun Sun View Article
human	WB	Cell Rep METTL14 regulates chromatin bivalent domains in mouse embryonic stem cells Authors - Mandi Mu View Article
human	WB	Hum Cell MicroRNA-10a-5p-mediated downregulation of GATA6 inhibits tumor progression in ovarian cancer Authors - Feiying Gao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GATA6 Polyclonal antibody

GATA6 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Validation Data Gallery

WB analysis of MCF-7 using 55435-1-AP

WB analysis using 55435-1-AP

WB analysis of BxPC-3 using 55435-1-AP

IHC staining of human colon cancer using 55435-1-AP

IHC staining of human colon cancer using 55435-1-AP

IHC staining of rat liver using 55435-1-AP

IHC staining of rat liver using 55435-1-AP

FC experiment of HuH-7 using 55435-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling.

Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency

Pulmonary expression of CYP2A13 and ABCB1 is regulated by FOXA2 and their genetic interaction is associated with lung cancer.

ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4.

METTL14 regulates chromatin bivalent domains in mouse embryonic stem cells

MicroRNA-10a-5p-mediated downregulation of GATA6 inhibits tumor progression in ovarian cancer