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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GATAD2B Polyclonal antibody

GATAD2B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25679-1-AP

Synonyms

Transcriptional repressor p66-beta, P66beta, p66/p68, GATA zinc finger domain-containing protein 2B

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of A549 using 25679-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of MCF-7 using 25679-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of HeLa using 25679-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of PC-3 using 25679-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of HEK-293 using 25679-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    WB analysis of K-562 using 25679-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GATAD2B Polyclonal antibody (25679-1-AP)

    IF Staining of A431 using 25679-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using GATAD2B antibody (25679-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells, PC-3 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25679-1-AP targets GATAD2B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GATAD2B fusion protein Ag22615 相同性解析による交差性が予測される生物種
Full Name GATA zinc finger domain containing 2B
Observed molecular weight 69 kDa
GenBank accession numberBC069419
Gene symbol GATAD2B
Gene ID (NCBI) 57459
RRIDAB_2880190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GATAD2B antibody 25679-1-APDownload protocol
IF protocol for GATAD2B antibody 25679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila
View Article
mouseWB,IP

Cell Prolif

GATAD2B is required for pre-implantation embryonic development by regulating zygotic genome activation

Authors - Yuling Lin
View Article
humanWB

Nucleic Acids Res

CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development

Authors - Xue Chen
View Article