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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GATC Monoclonal antibody, PBS Only

GATC Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F9C8

Cat no : 68405-1-PBS

Synonyms

Protein 15E1.2, Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial, Glu-AdT subunit C, GatC like protein, EC:6.3.5.-

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using GATC Monoclonal antibody (68405-1-Ig)

    WB analysis of A549 using 68405-1-Ig (same clone as 68405-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GATC Monoclonal antibody (68405-1-Ig)

    WB analysis using 68405-1-Ig (same clone as 68405-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68405-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using GATC Monoclonal antibody (68405-1-Ig)

    IF Staining of HEK-293 using 68405-1-Ig (same clone as 68405-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GATC antibody (68405-1-Ig, Clone: 2F9C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68405-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68405-1-PBS targets GATC in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GATC fusion protein Ag19857 相同性解析による交差性が予測される生物種
Full Name glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial)
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC107145
Gene symbol GATC
Gene ID (NCBI) 283459
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).