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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBA Polyclonal antibody (27972-1-AP)

    WB analysis using 27972-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27972-1-AP (GBA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using GBA Polyclonal antibody (27972-1-AP)

    WB analysis of SH-SY5Y using 27972-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27972-1-AP (GBA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GBA Polyclonal antibody (27972-1-AP)

    IHC staining of mouse brain using 27972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GBA Polyclonal antibody (27972-1-AP)

    IHC staining of mouse brain using 27972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GBA Polyclonal antibody (27972-1-AP)

    IHC staining of mouse kidney using 27972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GBA Polyclonal antibody (27972-1-AP)

    IHC staining of mouse kidney using 27972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GBA Polyclonal antibody

GBA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27972-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

GBA1, Cholesteryl-beta-glucosidase, Cholesterol glucosyltransferase, Beta-glucosylceramidase 1, Beta-glucocerebrosidase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells
Positive IHC detected inmouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

27972-1-AP targets GBA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBA fusion protein Ag27388 相同性解析による交差性が予測される生物種
Full Name glucosidase, beta; acid (includes glucosylceramidase)
Calculated molecular weight 60 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC003356
Gene Symbol GBA
Gene ID (NCBI) 2629
RRIDAB_2881024
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04062
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GBA antibody 27972-1-APDownload protocol
IHC protocol for GBA antibody 27972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins

Authors - Fatema Akter
View Article
mouseWB

Sci Total Environ

Manganese disrupts the maturation and degradation of axonal autophagosome leading to hippocampal synaptic toxicity in mice via the activation of LRRK2 on phosphorylation of Rab10

Authors - Zhuo Ma
View Article