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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GBA Polyclonal antibody

GBA Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20622-1-AP

Synonyms

GBA1LP, glucosylceramidase beta 1 like, pseudogene, GBAP1, GBAP, Beta-GC

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GBA Polyclonal antibody (20622-1-AP)

    IHC staining of human breast cancer using 20622-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GBA Polyclonal antibody (20622-1-AP)

    IHC staining of human breast cancer using 20622-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using GBA Polyclonal antibody (20622-1-AP)

    IHC staining of human brain using 20622-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using GBA Polyclonal antibody (20622-1-AP)

    IHC staining of human brain using 20622-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using GBA Polyclonal antibody (20622-1-AP)

    IF Staining of SH-SY5Y using 20622-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20622-1-AP (GBA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman breast cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20622-1-AP targets GBA in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBA fusion protein Ag13981 相同性解析による交差性が予測される生物種
Full Name glucosidase, beta; acid, pseudogene
Calculated molecular weight 321 aa, 36 kDa
GenBank accession numberBC000349
Gene symbol GBA
Gene ID (NCBI) 2630
RRIDAB_10696317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for GBA antibody 20622-1-APDownload protocol
IF protocol for GBA antibody 20622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols