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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GBAS Polyclonal antibody

GBAS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18570-1-AP

Synonyms

GBAS, NIPSNAP2, Protein NipSnap homolog 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBAS Polyclonal antibody (18570-1-AP)

    WB analysis using 18570-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18570-1-AP (GBAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GBAS Polyclonal antibody (18570-1-AP)

    WB analysis using 18570-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18570-1-AP (GBAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GBAS Polyclonal antibody (18570-1-AP)

    WB analysis of HepG2 using 18570-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18570-1-AP (GBAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using GBAS Polyclonal antibody (18570-1-AP)

    IHC staining of human heart using 18570-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18570-1-AP (GBAS Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GBAS Polyclonal antibody (18570-1-AP)

    IHC staining of human heart using 18570-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18570-1-AP (GBAS Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inA549 cells, mouse brain tissue, HepG2 cells, rat brain tissue, T-47D cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18570-1-AP targets GBAS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBAS fusion protein Ag13405 相同性解析による交差性が予測される生物種
Full Name glioblastoma amplified sequence
Calculated molecular weight 34 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC030821
Gene symbol GBAS
Gene ID (NCBI) 2631
RRIDAB_2878553
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GBAS antibody 18570-1-APDownload protocol
IHC protocol for GBAS antibody 18570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols