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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBE1 Recombinant antibody (85143-5-RR)

    WB analysis using 85143-5-RR (same clone as 85143-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85143-5-RR (GBE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85143-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GBE1 Recombinant antibody (85143-5-RR)

    WB analysis using 85143-5-RR (same clone as 85143-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85143-5-RR (GBE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85143-5-PBS in a different storage buffer formulation.

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GBE1 Recombinant antibody, PBS Only

GBE1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85143-5-PBS
Clone No.242705G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Glycogen-branching enzyme, GBE, EC:2.4.1.18, APBD, 242705G3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85143-5-PBS targets GBE1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GBE1 fusion protein Ag14154 相同性解析による交差性が予測される生物種
Full Name glucan (1,4-alpha-), branching enzyme 1
Calculated molecular weight 702 aa, 80 kDa
Observed molecular weight72 kDa
GenBank accession numberBC012098
Gene Symbol GBE1
Gene ID (NCBI) 2632
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04446
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GBE1 (glucan (1,4-alpha-), branching enzyme 1) is essential for the globular and branched structure of glycogen, increasing solubility by creating a hydrophilic surface and reducing intracellular osmotic pressure. It may serve as a potential therapeutic target for treating LUAD(PMID: 31221150). The full sequence can be further processed into a mature form of 75 kDa.