Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
25183-1-AP targets GBF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GBF1 fusion protein Ag16579 相同性解析による交差性が予測される生物種 |
| Full Name | golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1 |
| Calculated molecular weight | 1859 aa, 206 kDa |
| Observed molecular weight | 206 kDa |
| GenBank accession number | BC117681 |
| Gene Symbol | GBF1 |
| Gene ID (NCBI) | 8729 |
| RRID | AB_2879948 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92538 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GBF1 antibody 25183-1-AP | Download protocol |
| IHC protocol for GBF1 antibody 25183-1-AP | Download protocol |
| IF protocol for GBF1 antibody 25183-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Pathog Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. | ||
Sci Rep The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.
| ||
J Virol Enterovirus 3A protein disrupts endoplasmic reticulum homeostasis through interaction with GBF1 |