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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GBP1 Monoclonal antibody (67161-1-Ig)

    WB analysis of HeLa using 67161-1-Ig (same clone as 67161-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using GBP1 Monoclonal antibody (67161-1-Ig)

    WB analysis of HUVEC using 67161-1-Ig (same clone as 67161-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GBP1 Monoclonal antibody (67161-1-Ig)

    WB analysis of HEK-293 using 67161-1-Ig (same clone as 67161-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using GBP1 Monoclonal antibody (67161-1-Ig)

    WB analysis of human placenta using 67161-1-Ig (same clone as 67161-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using GBP1 Monoclonal antibody (67161-1-Ig)

    WB analysis of pig spleen using 67161-1-Ig (same clone as 67161-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using GBP1 Monoclonal antibody (67161-1-Ig)

    WB analysis of rat spleen using 67161-1-Ig (same clone as 67161-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GBP1 Monoclonal antibody (67161-1-Ig)

    IHC staining of human ovary tumor using 67161-1-Ig (same clone as 67161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GBP1 Monoclonal antibody (67161-1-Ig)

    IHC staining of human ovary tumor using 67161-1-Ig (same clone as 67161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

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GBP1 Monoclonal antibody, PBS Only

GBP1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67161-1-PBS
Clone No.2A10E8

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1, EC:3.6.1.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67161-1-PBS targets GBP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GBP1 fusion protein Ag7767 相同性解析による交差性が予測される生物種
Full Name GTP binding protein 1
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC002666
Gene Symbol GBP1
Gene ID (NCBI) 2633
RRIDAB_2882457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP32455
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II IFNs and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.