• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GBP1 Recombinant antibody, PBS Only

GBP1 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240634G8

Cat no : 83751-6-PBS

Synonyms

Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1, EC:3.6.1.-

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GBP1 Recombinant antibody (83751-6-RR)

    WB analysis using 83751-6-RR (same clone as 83751-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83751-6-RR (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83751-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using GBP1 Recombinant antibody (83751-6-RR)

    FC experiment of HeLa using 83751-6-RR (same clone as 83751-6-PBS)

    1x10^6 HeLa cells untreated (dashed lines) or treated with IFN gamma (red) were intracellularly stained with 0.25 ug GBP1 Recombinant antibody (83751-6-RR, Clone: 240634G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83751-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83751-6-RR

    Affinity and Kinetic Characterization of 83751-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83751-6-RR against Human GBP1 were performed. The affinity constant is below 1 pM.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83751-6-PBS is GBP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GBP1 fusion protein Ag7562 相同性解析による交差性が予測される生物種
Full Name GTP binding protein 1
Calculated molecular weight 68 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC002666
Gene symbol GBP1
Gene ID (NCBI) 2633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by interferon and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II interferons and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus, and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.