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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis using 11854-1-AP (same clone as 11854-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis using 11854-1-AP (same clone as 11854-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis of K-562 using 11854-1-AP (same clone as 11854-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human lung tissue using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis of human lung using 11854-1-AP (same clone as 11854-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis of human placenta using 11854-1-AP (same clone as 11854-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human spleen using 11854-1-AP (same clone as 11854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human spleen using 11854-1-AP (same clone as 11854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human tonsillitis using 11854-1-AP (same clone as 11854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human tonsillitis using 11854-1-AP (same clone as 11854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GBP2 Polyclonal antibody (11854-1-AP)

    IF Staining of HeLa using 11854-1-AP (same clone as 11854-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBP2 antibody (11854-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GBP2 Polyclonal antibody, PBS Only

GBP2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11854-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11854-1-PBS targets GBP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP2 fusion protein Ag2425 相同性解析による交差性が予測される生物種
Full Name guanylate binding protein 2, interferon-inducible
Calculated molecular weight 591 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC022272
Gene Symbol GBP2
Gene ID (NCBI) 2634
RRIDAB_2109336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32456
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)