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  • Featured Product
  • KD/KO Validated

GBP2 Polyclonal antibody, PBS Only

GBP2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11854-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis using 11854-1-AP (same clone as 11854-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis using 11854-1-AP (same clone as 11854-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis of K-562 using 11854-1-AP (same clone as 11854-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis of human placenta using 11854-1-AP (same clone as 11854-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human lung tissue using GBP2 Polyclonal antibody (11854-1-AP)

    WB analysis of human lung using 11854-1-AP (same clone as 11854-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human spleen using 11854-1-AP (same clone as 11854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human spleen using 11854-1-AP (same clone as 11854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human tonsillitis using 11854-1-AP (same clone as 11854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GBP2 Polyclonal antibody (11854-1-AP)

    IHC staining of human tonsillitis using 11854-1-AP (same clone as 11854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GBP2 Polyclonal antibody (11854-1-AP)

    IF Staining of HeLa using 11854-1-AP (same clone as 11854-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBP2 antibody (11854-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11854-1-PBS targets GBP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP2 fusion protein Ag2425 相同性解析による交差性が予測される生物種
Full Name guanylate binding protein 2, interferon-inducible
Calculated molecular weight 591 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC022272
Gene symbol GBP2
Gene ID (NCBI) 2634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)