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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBP3 Polyclonal antibody (30719-1-AP)

    WB analysis using 30719-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30719-1-AP (GBP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GBP3 Polyclonal antibody (30719-1-AP)

    IHC staining of mouse cerebellum using 30719-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GBP3 Polyclonal antibody (30719-1-AP)

    IHC staining of mouse cerebellum using 30719-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using GBP3 Polyclonal antibody (30719-1-AP)

    IHC staining of mouse lung using 30719-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using GBP3 Polyclonal antibody (30719-1-AP)

    IHC staining of mouse lung using 30719-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GBP3 Polyclonal antibody

GBP3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30719-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Guanine nucleotide-binding protein 3, GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHUVEC cells
Positive IHC detected inmouse cerebellum tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30719-1-AP targets GBP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP3 fusion protein Ag33889 相同性解析による交差性が予測される生物種
Full Name guanylate binding protein 3
Observed molecular weight68 kDa
GenBank accession numberBC140837
Gene Symbol GBP3
Gene ID (NCBI) 2635
RRIDAB_3086400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0R5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanylate-binding protein 3 (GBP3) is involved in the proliferation of glioma cells through regulating SQSTM1-ERK1/2 pathway. GBP3 has 2 isoforms, one is 595 amino acids long at 68 kDa and the other is at nearly 62 kDa.

Protocols

Product Specific Protocols
WB protocol for GBP3 antibody 30719-1-APDownload protocol
IHC protocol for GBP3 antibody 30719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols