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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GBP4 Polyclonal antibody

GBP4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17746-1-AP

Synonyms

Mpa2, Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBP4 Polyclonal antibody (17746-1-AP)

    WB analysis using 17746-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17746-1-AP (GBP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using GBP4 Polyclonal antibody (17746-1-AP)

    WB analysis of human placenta using 17746-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 17746-1-AP (GBP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using GBP4 Polyclonal antibody (17746-1-AP)

    WB analysis of A431 using 17746-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 17746-1-AP (GBP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using GBP4 Polyclonal antibody (17746-1-AP)

    IP experiment of A431 using 17746-1-AP

    IP result of anti-GBP4 (IP:17746-1-AP, 4ug; Detection:17746-1-AP 1:500) with A431 cells lysate 1040 ug.

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Tested Applications

Positive WB detected inHeLa cells, human placenta tissue, A431 cells, mouse heart tissue
Positive IP detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17746-1-AP targets GBP4 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP4 fusion protein Ag12006 相同性解析による交差性が予測される生物種
Full Name guanylate binding protein 4
Calculated molecular weight 640 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC070055
Gene symbol GBP4
Gene ID (NCBI) 115361
RRIDAB_2109346
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor. The molecular weight of GBP4 is 73 kDa.

Protocols

Product Specific Protocols
WB protocol for GBP4 antibody 17746-1-APDownload protocol
IP protocol for GBP4 antibody 17746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

mBio

Human GBP1 facilitates the rupture of the Legionella-containing vacuole and inflammasome activation

Authors - Antonia R Bass
View Article