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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Grancalcin Polyclonal antibody (15787-1-AP)

    WB analysis using 15787-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15787-1-AP (Grancalcin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using Grancalcin Polyclonal antibody (15787-1-AP)

    WB analysis of HL-60 using 15787-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15787-1-AP (Grancalcin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Grancalcin Polyclonal antibody

Grancalcin Polyclonal Antibody for WB, ELISA
Cat No. 15787-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GCA, GCL, Grancalcin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HL-60 cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15787-1-AP targets Grancalcin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Grancalcin fusion protein Ag8486 相同性解析による交差性が予測される生物種
Full Name grancalcin, EF-hand calcium binding protein
Calculated molecular weight 217 aa, 24 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC005214
Gene Symbol Grancalcin
Gene ID (NCBI) 25801
RRIDAB_2878185
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28676
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Grancalcin antibody 15787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols