Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse ovary tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IF | See 3 publications below |
Product Information
16271-1-AP targets GCC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GCC1 fusion protein Ag9339 相同性解析による交差性が予測される生物種 |
| Full Name | GRIP and coiled-coil domain containing 1 |
| Calculated molecular weight | 775 aa, 88 kDa |
| Observed molecular weight | 105 kDa |
| GenBank accession number | BC014100 |
| Gene Symbol | GCC1 |
| Gene ID (NCBI) | 79571 |
| RRID | AB_2107197 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96CN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GCC1 (Golgi coiled coil protein 1), also known as GCC1P or GCC88, is a protein probably involved in maintaining Golgi structure. The MW of this protein is 105 kDa, and Catalog#16271-1-AP specially recognises the 105 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GCC1 antibody 16271-1-AP | Download protocol |
| IHC protocol for GCC1 antibody 16271-1-AP | Download protocol |
| IF protocol for GCC1 antibody 16271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin. | ||
Mol Biol Cell Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking. | ||
bioRxiv SARS-CoV-2 triggers Golgi fragmentation via down-regulation of GRASP55 to facilitate viral trafficking. | ||