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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using GCET2 Polyclonal antibody (13447-1-AP)

    WB analysis of Raji using 13447-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 13447-1-AP (GCET2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using GCET2 Polyclonal antibody (13447-1-AP)

    IF Staining of Raji using 13447-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using GCET2 antibody (13447-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

GCET2 Polyclonal antibody

GCET2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13447-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Germinal center-associated signaling and motility protein, Germinal center-associated lymphoma protein, Germinal center B-cell-expressed transcript 2 protein, GCSAM, GCAT2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13447-1-AP targets GCET2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCET2 fusion protein Ag4280 相同性解析による交差性が予測される生物種
Full Name germinal center expressed transcript 2
Calculated molecular weight 178 aa, 21 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC030506
Gene Symbol GCET2
Gene ID (NCBI) 257144
RRIDAB_2877949
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6F7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GCET2, also named HGAL, is a newly gene that has been shown to be a useful marker for germinal centre (GC) B cells and GC B-cell derived malignancies, including follicular lymphomas and germinal centre B cell-like diffuse large B-cell lymphomas (GCB-DLBCLs), and is a useful prognosticator for DLBCLs. GCET2 may be an adaptor protein in GC B cells that transduces signals from GC B-cell membrane to the cytosol via its association with GRB2.

Protocols

Product Specific Protocols
WB protocol for GCET2 antibody 13447-1-APDownload protocol
IF protocol for GCET2 antibody 13447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols