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GCH1 Polyclonal antibody
GCH1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 28501-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | rat liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IF | See 2 publications below |
Product Information
28501-1-AP targets GCH1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GCH1 fusion protein Ag28896 相同性解析による交差性が予測される生物種 |
| Full Name | GTP cyclohydrolase 1 |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC025415 |
| Gene symbol | GCH1 |
| Gene ID (NCBI) | 2643 |
| RRID | AB_2881157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GCH1 antibody 28501-1-AP | Download protocol |
| IHC protocol for GCH1 antibody 28501-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Free Radic Res 3,6-Epidioxy-1,10-bisaboladiene induces ferroptosis-like cell death through lipid peroxidation | ||
Nat Cell Biol Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition | ||
Int J Oncol Long non‑coding RNA lung cancer‑associated transcript 1 regulates ferroptosis via microRNA‑34a‑5p‑mediated GTP cyclohydrolase 1 downregulation in lung cancer cells | ||
Neuron Spliceosomal GTPase Eftud2 deficiency-triggered ferroptosis leads to Purkinje cell degeneration | ||
Eur Rev Med Pharmacol Sci Identification of ferroptosis-related genes for the prediction of prognosis and chemotherapy benefit of gastric cancer | ||
Virol Sin Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1
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