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  • Featured Product
  • KD/KO Validated

GCK Polyclonal antibody, PBS Only

GCK Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, pig

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15629-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCK Polyclonal antibody (15629-1-AP)

    WB analysis using 15629-1-AP (same clone as 15629-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15629-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GCK Polyclonal antibody (15629-1-AP)

    WB analysis using 15629-1-AP (same clone as 15629-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15629-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GCK Polyclonal antibody (15629-1-AP)

    IP experiment of mouse liver using 15629-1-AP (same clone as 15629-1-PBS)

    IP result of anti-GCK (IP:15629-1-AP, 4ug; Detection:15629-1-AP 1:500) with mouse liver tissue lysate 7000ug. This data was developed using the same antibody clone with 15629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCK Polyclonal antibody (15629-1-AP)

    IHC staining of human liver cancer using 15629-1-AP (same clone as 15629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCK Polyclonal antibody (15629-1-AP)

    IHC staining of human liver cancer using 15629-1-AP (same clone as 15629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using GCK Polyclonal antibody (15629-1-AP)

    IHC staining of rat liver using 15629-1-AP (same clone as 15629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using GCK Polyclonal antibody (15629-1-AP)

    IHC staining of rat liver using 15629-1-AP (same clone as 15629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15629-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15629-1-PBS targets GCK in WB, IHC, IP, Indirect ELISA applications and shows reactivity with mouse, rat, pig samples.

Tested Reactivity mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCK fusion protein Ag7904 相同性解析による交差性が予測される生物種
Full Name glucokinase (hexokinase 4)
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC001890
Gene symbol GCK
Gene ID (NCBI) 2645
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa. The westernblotting results of human cells we tested are not well, therefore, we do not recommend it to customers who would like to do WB involved in human.