Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 14241-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14241-1-AP (GCLM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14241-1-AP
WB result of GCLM antibody (14241-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HeLa cells.
WB analysis using 14241-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14241-1-AP (GCLM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 14241-1-AP
IP result of anti-GCLM (IP:14241-1-AP, 4ug; Detection:14241-1-AP 1:2000) with A431 cells lysate 1000 ug.
IHC staining of human liver cancer using 14241-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14241-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 14241-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 14241-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14241-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14241-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 14241-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14241-1-AP (GCLM antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 14241-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14241-1-AP (GCLM antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 14241-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GCLM antibody (14241-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 14241-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GCLM Polyclonal antibody (14241-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

GCLM Polyclonal antibody

GCLM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14241-1-AP

Publications(152)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GLCLR, Glutamate--cysteine ligase modifier subunit, Glutamate--cysteine ligase regulatory subunit

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (152)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, L02 cells, mouse liver tissue, rat liver tissue, HepG2 cells
Positive IP detected in	A431 cells
Positive IHC detected in	human liver cancer tissue, human skeletal muscle tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 144 publications below
IHC	See 10 publications below
IF	See 6 publications below

Product Information

14241-1-AP targets GCLM in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5497 Product name: Recombinant human GCLM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-274 aa of BC041809 Sequence: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS 相同性解析による交差性が予測される生物種
Full Name	glutamate-cysteine ligase, modifier subunit
Calculated molecular weight	31 kDa
Observed molecular weight	31 kDa
GenBank accession number	BC041809
Gene Symbol	GCLM
Gene ID (NCBI)	2730
RRID	AB_2107832
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P48507
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.

Protocols

Product Specific Protocols
IF protocol for GCLM antibody 14241-1-AP	Download protocol
IHC protocol for GCLM antibody 14241-1-AP	Download protocol
IP protocol for GCLM antibody 14241-1-AP	Download protocol
WB protocol for GCLM antibody 14241-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Crit Care Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein. Authors - Lingbing Zhang View Article
mouse	WB	ACS Cent Sci Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury Authors - Juan Zhang View Article
mouse	WB	Hepatology Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury. Authors - Yu Sun View Article
mouse	WB	Acta Pharm Sin B Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis Authors - Yujun Zhou View Article
human	WB	J Clin Invest Identification of CD84 as a potent survival factor in acute myeloid leukemia Authors - Yinghui Zhu View Article
mouse	WB	Sci Adv Immunomodulatory actions of a kynurenine-derived endogenous electrophile. Authors - Mara Carreño View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14241-1-AP

WB analysis of HeLa using 14241-1-AP

WB analysis using 14241-1-AP

IP experiment of A431 using 14241-1-AP

IHC staining of human liver cancer using 14241-1-AP

IHC staining of human liver cancer using 14241-1-AP

IHC staining of mouse skeletal muscle using 14241-1-AP

IHC staining of mouse skeletal muscle using 14241-1-AP

IHC staining of human liver cancer using 14241-1-AP

IHC staining of human liver cancer using 14241-1-AP

IHC staining of human skeletal muscle using 14241-1-AP

IHC staining of human skeletal muscle using 14241-1-AP

IF Staining of HepG2 using 14241-1-AP

FC experiment of HepG2 using 14241-1-AP

GCLM Polyclonal antibody

GCLM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.

Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis

Identification of CD84 as a potent survival factor in acute myeloid leukemia

Immunomodulatory actions of a kynurenine-derived endogenous electrophile.