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  • Featured Product
  • KD/KO Validated

GCLM Monoclonal antibody, PBS Only

GCLM Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A5A4

Cat no : 66808-1-PBS

Synonyms

Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCLM Monoclonal antibody (66808-1-Ig)

    WB analysis using 66808-1-Ig (same clone as 66808-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66808-1-Ig (GCLM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GCLM Monoclonal antibody (66808-1-Ig)

    WB analysis of HEK-293 using 66808-1-Ig (same clone as 66808-1-PBS)

    WB result of GCLM antibody (66808-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HEK-293 cells. This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human lung cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human lung cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66808-1-PBS targets GCLM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GCLM fusion protein Ag5497 相同性解析による交差性が予測される生物種
Full Name glutamate-cysteine ligase, modifier subunit
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC041809
Gene symbol GCLM
Gene ID (NCBI) 2730
RRIDAB_2882151
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.