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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCLM Monoclonal antibody (66808-1-Ig)

    WB analysis using 66808-1-Ig (same clone as 66808-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66808-1-Ig (GCLM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GCLM Monoclonal antibody (66808-1-Ig)

    WB analysis of HEK-293 using 66808-1-Ig (same clone as 66808-1-PBS)

    WB result of GCLM antibody (66808-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HEK-293 cells. This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human lung cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human lung cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Monoclonal antibody (66808-1-Ig)

    IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GCLM Monoclonal antibody, PBS Only

GCLM Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66808-1-PBS
Clone No.1A5A4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66808-1-PBS targets GCLM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GCLM fusion protein Ag5497 相同性解析による交差性が予測される生物種
Full Name glutamate-cysteine ligase, modifier subunit
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC041809
Gene Symbol GCLM
Gene ID (NCBI) 2730
RRIDAB_2882151
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48507
Storage Buffer PBS only
Storage ConditionsStore at -80°C.