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  • Featured Product
  • KD/KO Validated

GCLM Recombinant antibody, PBS Only

GCLM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4K16

Cat no : 82623-1-PBS

Synonyms

Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCLM Recombinant antibody (82623-1-RR)

    WB analysis using 82623-1-RR (same clone as 82623-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GCLM Recombinant antibody (82623-1-RR)

    WB analysis of HeLa using 82623-1-RR (same clone as 82623-1-PBS)

    WB result of GCLM antibody (82623-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HeLa cells. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using GCLM Recombinant antibody (82623-1-RR)

    WB analysis of RAW 264.7 using 82623-1-RR (same clone as 82623-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of mouse brain using 82623-1-RR (same clone as 82623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of mouse brain using 82623-1-RR (same clone as 82623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of human ovarian cancer using 82623-1-RR (same clone as 82623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of human ovarian cancer using 82623-1-RR (same clone as 82623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of human skin cancer using 82623-1-RR (same clone as 82623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of human skin cancer using 82623-1-RR (same clone as 82623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82623-1-RR

    Affinity and Kinetic Characterization of 82623-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82623-1-RR against Human GCLM were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82623-1-PBS targets GCLM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GCLM fusion protein Ag5497 相同性解析による交差性が予測される生物種
Full Name glutamate-cysteine ligase, modifier subunit
Calculated molecular weight 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC041809
Gene symbol GCLM
Gene ID (NCBI) 2730
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.