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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GCM1 Polyclonal antibody

GCM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21724-1-AP

Synonyms

hGCMa, Glial cells missing homolog 1, GCMA, GCM motif protein 1, Chorion-specific transcription factor GCMa

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GCM1 Polyclonal antibody (21724-1-AP)

    WB analysis of HepG2 using 21724-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21724-1-AP (GCM1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GCM1 Polyclonal antibody (21724-1-AP)

    WB analysis of HeLa using 21724-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21724-1-AP (GCM1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using GCM1 Polyclonal antibody (21724-1-AP)

    IHC staining of human placenta using 21724-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21724-1-AP (GCM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using GCM1 Polyclonal antibody (21724-1-AP)

    IHC staining of human placenta using 21724-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21724-1-AP (GCM1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

21724-1-AP targets GCM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCM1 fusion protein Ag16383 相同性解析による交差性が予測される生物種
Full Name glial cells missing homolog 1 (Drosophila)
Calculated molecular weight 436 aa, 49 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC096288
Gene symbol GCM1
Gene ID (NCBI) 8521
RRIDAB_2878913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GCM1 antibody 21724-1-APDownload protocol
IHC protocol for GCM1 antibody 21724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Reprod Biol Endocrinol

Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.

Authors - Wei Peng
View Article
humanWB

BMC Med

Epigenetic drug screening for trophoblast syncytialization reveals a novel role for MLL1 in regulating fetoplacental growth

Authors - Jiayi Wu
View Article