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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCNA Polyclonal antibody (25516-1-AP)

    WB analysis using 25516-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 25516-1-AP (GCNA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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GCNA Polyclonal antibody

GCNA Polyclonal Antibody for WB, ELISA
Cat No. 25516-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

acidic repeat containing, ACRC, GCNA, NAAR1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25516-1-AP targets GCNA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCNA fusion protein Ag21895 相同性解析による交差性が予測される生物種
Full Name acidic repeat containing
Calculated molecular weight 691 aa, 76 kDa
Observed molecular weight90 kDa
GenBank accession numberBC136259
Gene Symbol GCNA
Gene ID (NCBI) 93953
RRIDAB_3085801
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GCNA, also known as ACRC, belongs to the serine-aspartate repeat-containing protein (SDr) family. GCNA has remained tightly associated with sexual reproduction showing enriched expression within germ cells in both invertebrate and vertebrate species. GCNA has recently been implicated in resolving DPCs in germ cells and early embryos. GCNA is targeted to SUMOylated DPC proteins through its SUMO-interacting motifs (SIMs), reinforcing the importance of SUMOylation in DPC repair. The amino acid sequence homology of GCNA in humans and mice is very low. The calculated molecular weight of GCNA is 76 kDa. Sometimes higher molecular weight around 90 kDa can also be observed, which may be caused by SUMO modification (PMID: 31839537, 32329115).

Protocols

Product Specific Protocols
WB protocol for GCNA antibody 25516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols