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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GCNA Polyclonal antibody

GCNA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30693-1-AP

Synonyms

acidic repeat containing, ACRC, GCNA, NAAR1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCNA Polyclonal antibody (30693-1-AP)

    WB analysis using 30693-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30693-1-AP (ACRC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GCNA Polyclonal antibody (30693-1-AP)

    WB analysis using 30693-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30693-1-AP (GCNA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inLNCaP cells, PC-3 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30693-1-AP targets GCNA in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCNA fusion protein Ag33720 相同性解析による交差性が予測される生物種
Full Name acidic repeat containing
Calculated molecular weight 691 aa, 76 kDa
Observed molecular weight76 kDa
GenBank accession numberBC136259
Gene symbol GCNA
Gene ID (NCBI) 93953
RRIDAB_3086389
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Germ cell nuclear acidic protein (GCNA) has been extensively used as a germ cell marker for almost 30 years. Recently, mutations in GCNA have been linked to azoospermia in humans, defining GCNA as a clinical determinant of human infertility.

Protocols

Product Specific Protocols
WB protocol for GCNA antibody 30693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols