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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GCNT1 Recombinant antibody

GCNT1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240071E9

Cat no : 83121-4-RR

Synonyms

Core 2 beta-1,6-N-acetylglucosaminyltransferase, C2GNT-L, C2GNT1, C2GlcNAcT, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCNT1 Recombinant antibody (83121-4-RR)

    WB analysis using 83121-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83121-4-RR (GCNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83121-4-RR

    Affinity and Kinetic Characterization of 83121-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83121-4-RR against Human GCNT1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inLNCaP cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83121-4-RR is GCNT1 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GCNT1 fusion protein Ag12264 相同性解析による交差性が予測される生物種
Full Name glucosaminyl (N-acetyl) transferase 1, core 2 (beta-1,6-N-acetylglucosaminyltransferase)
Calculated molecular weight 428 aa, 50 kDa
Observed molecular weight50 kDa
GenBank accession numberBC074885
Gene symbol GCNT1
Gene ID (NCBI) 2650
RRIDAB_3670831
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucosaminyl (N-acetyl) transferase 1 (GCNT1, also known as C2GNT1), is a glycosyltransferase enzyme that plays an essential role in the formation of core 2 branched O-glycans that is crucial to the final definition of O-glycan structure (PMID: 37813880). GCNT1 promotes the growth of prostate tumors and regulates oncogenic gene expression pathways that are important for disease progression (PMID: 24854630).

Protocols

Product Specific Protocols
WB protocol for GCNT1 antibody 83121-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols