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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GCSH Recombinant antibody, PBS Only (Capture/Detector)

GCSH Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230505C4

Cat no : 83012-2-PBS

Synonyms

NKH, MMDS7, Glycine cleavage system H protein, mitochondrial, GCE, 230505C4

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00021-3

    Sandwich ELISA standard curve of MP00021-3

    Sandwich ELISA standard curve of MP00021-3, Human GCSH Recombinant Matched Antibody Pair - PBS only. 83012-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10174. 83012-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP00021-3

    Sample test of MP00021-3

    The mean GCSH concentration was determined to be 24.39 ng/mL in HeLa cell extract based on a 3.60 mg/mL extract load and 23.77 ng/mL in HEK-293 cell extract based on a 3.40 mg/mL extract load.

  • Cytometric bead array standard curve of MP00021-3

    Cytometric bead array standard curve of MP00021-3

    Cytometric bead array standard curve of MP00021-3, GCSH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83012-3-PBS. Detection antibody: 83012-2-PBS. Standard: Ag10174. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00021-1

    Cytometric bead array standard curve of MP00021-1

    Cytometric bead array standard curve of MP00021-1, GCSH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83012-2-PBS. Detection antibody: 83012-1-PBS. Standard: Ag10174. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00021-2

    Cytometric bead array standard curve of MP00021-2

    Cytometric bead array standard curve of MP00021-2, GCSH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83012-2-PBS. Detection antibody: 83012-4-PBS. Standard: Ag10174. Range: 0.625-20 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83012-2-PBS targets GCSH as part of a matched antibody pair:

MP00021-1: 83012-2-PBS capture and 83012-1-PBS detection (validated in Cytometric bead array)

MP00021-2: 83012-2-PBS capture and 83012-4-PBS detection (validated in Cytometric bead array)

MP00021-3: 83012-3-PBS capture and 83012-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GCSH fusion protein Ag10174 相同性解析による交差性が予測される生物種
Full Name glycine cleavage system protein H (aminomethyl carrier)
Calculated molecular weight 19 kDa
GenBank accession numberBC000790
Gene symbol GCSH
Gene ID (NCBI) 2653
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.