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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GDAP1 Polyclonal antibody

GDAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13152-1-AP

Synonyms

CMTRIA, GDAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GDAP1 Polyclonal antibody (13152-1-AP)

    WB analysis using 13152-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using GDAP1 Polyclonal antibody (13152-1-AP)

    WB analysis of fetal human brain using 13152-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse brain tissue using GDAP1 Polyclonal antibody (13152-1-AP)

    WB analysis of mouse brain using 13152-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of human brain tissue using GDAP1 Polyclonal antibody (13152-1-AP)

    WB analysis of human brain using 13152-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GDAP1 Polyclonal antibody (13152-1-AP)

    IHC staining of mouse brain using 13152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GDAP1 Polyclonal antibody (13152-1-AP)

    IHC staining of mouse brain using 13152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSH-SY5Y cells, human brain tissue, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13152-1-AP targets GDAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GDAP1 fusion protein Ag3769 相同性解析による交差性が予測される生物種
Full Name ganglioside-induced differentiation-associated protein 1
Calculated molecular weight 358 aa, 41 kDa
Observed molecular weight 36-41 kDa
GenBank accession numberBC024939
Gene symbol GDAP1
Gene ID (NCBI) 54332
RRIDAB_2232103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GDAP1 is a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy.

Protocols

Product Specific Protocols
WB protocol for GDAP1 antibody 13152-1-APDownload protocol
IHC protocol for GDAP1 antibody 13152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols