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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GDAP1 Monoclonal antibody

GDAP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3E10D4

Cat no : 68083-1-Ig

Synonyms

CMTRIA, GDAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GDAP1 Monoclonal antibody (68083-1-Ig)

    WB analysis using 68083-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68083-1-Ig (GDAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunohistochemistry (IHC) staining of rat brain tissue using GDAP1 Monoclonal antibody (68083-1-Ig)

    IHC staining of rat brain using 68083-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68083-1-Ig (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using GDAP1 Monoclonal antibody (68083-1-Ig)

    IHC staining of rat brain using 68083-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68083-1-Ig (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GDAP1 Monoclonal antibody (68083-1-Ig)

    IF Staining of mouse brain using 68083-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GDAP1 antibody (68083-1-Ig, Clone: 3E10D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GDAP1 Monoclonal antibody (68083-1-Ig)

    IF Staining of mouse brain using 68083-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GDAP1 antibody (68083-1-Ig, Clone: 3E10D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inpig brain tissue, Rabbit brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68083-1-Ig targets GDAP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GDAP1 fusion protein Ag28616 相同性解析による交差性が予測される生物種
Full Name ganglioside-induced differentiation-associated protein 1
Calculated molecular weight 358 aa, 41 kDa
Observed molecular weight38-40 kDa
GenBank accession numberBC024939
Gene symbol GDAP1
Gene ID (NCBI) 54332
RRIDAB_2918820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GDAP1 is a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy.

Protocols

Product Specific Protocols
WB protocol for GDAP1 antibody 68083-1-IgDownload protocol
IHC protocol for GDAP1 antibody 68083-1-IgDownload protocol
IF protocol for GDAP1 antibody 68083-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols