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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GDEP Polyclonal antibody

GDEP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22744-1-AP

Synonyms

GDEP, PCAN1

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using GDEP Polyclonal antibody (22744-1-AP)

    WB analysis of PC-3 using 22744-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22744-1-AP (GDEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GDEP Polyclonal antibody (22744-1-AP)

    IHC staining of human prostate cancer using 22744-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 22744-1-AP (GDEP Antibody) at dilution of 1:50. (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GDEP Polyclonal antibody (22744-1-AP)

    IHC staining of human prostate cancer using 22744-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 22744-1-AP (GDEP Antibody) at dilution of 1:50. (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GDEP Polyclonal antibody (22744-1-AP)

    IHC staining of human prostate cancer using 22744-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22744-1-AP (GDEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using GDEP Polyclonal antibody (22744-1-AP)

    IHC staining of human prostate hyperplasia using 22744-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia slide using 22744-1-AP (GDEP Antibody) at dilution of 1:50. (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using GDEP Polyclonal antibody (22744-1-AP)

    IHC staining of human prostate hyperplasia using 22744-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia slide using 22744-1-AP (GDEP Antibody) at dilution of 1:50. (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using GDEP Polyclonal antibody (22744-1-AP)

    IF Staining of PC-3 using 22744-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 22744-1-AP (GDEP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22744-1-AP is GDEP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GDEP fusion protein Ag19087 相同性解析による交差性が予測される生物種
Full Name gene differentially expressed in prostate
Calculated molecular weight 34 aa, 4 kDa
Observed molecular weight 4 kDa
GenBank accession numberBC125139
Gene symbol GDEP
Gene ID (NCBI) 118425
RRIDAB_2879161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GDEP antibody 22744-1-APDownload protocol
IHC protocol for GDEP antibody 22744-1-APDownload protocol
IF protocol for GDEP antibody 22744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols