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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GDF-15 Recombinant antibody (85100-1-RR)

    WB analysis using 85100-1-RR (same clone as 85100-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85100-1-RR (GDF15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85100-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GDF-15 Recombinant antibody (85100-1-RR)

    WB analysis using 85100-1-RR (same clone as 85100-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85100-1-RR (GDF-15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85100-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using GDF-15 Recombinant antibody (85100-1-RR)

    IHC staining of human placenta using 85100-1-RR (same clone as 85100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85100-1-RR (GDF-15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85100-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using GDF-15 Recombinant antibody (85100-1-RR)

    IHC staining of human placenta using 85100-1-RR (same clone as 85100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85100-1-RR (GDF-15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85100-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85100-1-RR

    Affinity and Kinetic Characterization of 85100-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85100-1-RR against Human GDF-15 were performed. The affinity constant is 39.3 nM.

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GDF-15 Recombinant antibody, PBS Only

GDF-15 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85100-1-PBS
Clone No.242173B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

GDF15, MIC-1, Growth/differentiation factor 15, growth differentiation factor 15, GDF 15

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85100-1-PBS targets GDF-15 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GDF-15 fusion protein Ag26790 相同性解析による交差性が予測される生物種
Full Name growth differentiation factor 15
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC008962
Gene Symbol GDF15
Gene ID (NCBI) 9518
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ99988
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.