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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GDF1 Polyclonal antibody (28345-1-AP)

    WB analysis using 28345-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28345-1-AP (GDF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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GDF1 Polyclonal antibody

GDF1 Polyclonal Antibody for WB, ELISA
Cat No. 28345-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

GDF 1, GDF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected infetal human brain tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28345-1-AP targets GDF1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GDF1 fusion protein Ag28831 相同性解析による交差性が予測される生物種
Full Name growth differentiation factor 1
Calculated molecular weight 39 kDa
Observed molecular weight39 kDa
GenBank accession numberNM_001492
Gene Symbol GDF1
Gene ID (NCBI) 2657
RRIDAB_3086045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP27539
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GDF1 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. Studies in rodents suggest that this protein is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis.

Protocols

Product Specific Protocols
WB protocol for GDF1 antibody 28345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols