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Validation Data Gallery

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  • Western Blot (WB) analysis of pig bone marrow tissue using GDF10 Monoclonal antibody (66371-1-Ig)

    WB analysis of pig bone marrow using 66371-1-Ig (same clone as 66371-1-PBS)

    pig bone marrow tissue were subjected to SDS PAGE followed by western blot with 66371-1-Ig (GDF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66371-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using GDF10 Monoclonal antibody (66371-1-Ig)

    WB analysis of ROS1728 using 66371-1-Ig (same clone as 66371-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66371-1-Ig (GDF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66371-1-PBS in a different storage buffer formulation.

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GDF10 Monoclonal antibody, PBS Only

GDF10 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66371-1-PBS
Clone No.1B7D12

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat

Applications

WB, Indirect ELISA

Synonyms

BIP, BMP 3b, BMP3B, Bone inducing protein, Bone morphogenetic protein 3B, GDF 10, GDF10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66371-1-PBS targets GDF10 in WB, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GDF10 fusion protein Ag4906 相同性解析による交差性が予測される生物種
Full Name growth differentiation factor 10
Calculated molecular weight 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC028237
Gene Symbol GDF10
Gene ID (NCBI) 2662
RRIDAB_2881750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55107
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Growth differentiation factor-10 (GDF10), also known as BMP3b, is a member of the transforming growth factor-b (TGF-b) superfamily. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This promotes neural repair after stroke. GDF10 plays a role in head formation and may have multiple roles in skeletal morphogenesis. Additionally, GDF10 may act as a tumor suppressor and reduced expression of this gene is associated with oral cancer.