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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GDF15 Polyclonal antibody (27845-1-AP)

    WB analysis using 27845-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27845-1-AP (GDF15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using GDF-15 Polyclonal antibody (27845-1-AP)

    IHC staining of human placenta using 27845-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27845-1-AP (GDF15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using GDF-15 Polyclonal antibody (27845-1-AP)

    IHC staining of human placenta using 27845-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27845-1-AP (GDF15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GDF-15 Polyclonal antibody

GDF-15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27845-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

GDF15, GDF 15, NAG-1, MIC-1, Growth/differentiation factor 15

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-1080 cells, HepG2 cells, LNCaP cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27845-1-AP targets GDF-15 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GDF-15 fusion protein Ag24090 相同性解析による交差性が予測される生物種
Full Name growth differentiation factor 15
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC008962
Gene Symbol GDF15
Gene ID (NCBI) 9518
RRIDAB_3669625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99988
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

Protocols

Product Specific Protocols
WB protocol for GDF-15 antibody 27845-1-APDownload protocol
IHC protocol for GDF-15 antibody 27845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols