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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GDF8/Myostatin Polyclonal antibody (19142-1-AP)

    WB analysis using 19142-1-AP (same clone as 19142-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using GDF8/Myostatin Polyclonal antibody (19142-1-AP)

    WB analysis of mouse heart using 19142-1-AP (same clone as 19142-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GDF8/Myostatin Polyclonal antibody (19142-1-AP)

    WB analysis of mouse liver using 19142-1-AP (same clone as 19142-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using GDF8/Myostatin Polyclonal antibody (19142-1-AP)

    WB analysis of PC-3 using 19142-1-AP (same clone as 19142-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using GDF8/Myostatin Polyclonal antibody (19142-1-AP)

    IHC staining of human heart using 19142-1-AP (same clone as 19142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using GDF8/Myostatin Polyclonal antibody (19142-1-AP)

    IHC staining of human heart using 19142-1-AP (same clone as 19142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GDF8/Myostatin Polyclonal antibody (19142-1-AP)

    IHC staining of mouse brain using 19142-1-AP (same clone as 19142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GDF8/Myostatin Polyclonal antibody, PBS Only

GDF8/Myostatin Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 19142-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19142-1-PBS targets GDF8/Myostatin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GDF8/Myostatin fusion protein Ag13605 相同性解析による交差性が予測される生物種
Full Name myostatin
Calculated molecular weight 375 aa, 43 kDa
Observed molecular weight 50 and 35 kDa
GenBank accession numberBC074757
Gene Symbol MSTN
Gene ID (NCBI) 2660
RRIDAB_10638615
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14793
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Myostatin, also known as GDF-8, is a member of the TGF-beta superfamily. Myostatin is expressed in the myotome and developing skeletal muscles. Myostatin negatively regulates skeletal muscle growth and development through the regulation of anabolic and catabolic pathways in skeletal muscles. Myostatin immunoreactive bands could be identified at ∼50, 32-35, and 16 kDa, which correspond to the precursor, glycosylated dimmer, and monomer, respectively (PMID: 17711997).