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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using GDI1 Polyclonal antibody (55214-1-AP)

    WB analysis of SH-SY5Y using 55214-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using GDI1 Polyclonal antibody (55214-1-AP)

    WB analysis of SH-SY5Y using 55214-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GDI1 Polyclonal antibody (55214-1-AP)

    WB analysis of human brain using 55214-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GDI1 Polyclonal antibody (55214-1-AP)

    WB analysis of mouse brain using 55214-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GDI1 Polyclonal antibody (55214-1-AP)

    WB analysis of mouse brain using 55214-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using GDI1 Polyclonal antibody (55214-1-AP)

    WB analysis of SH-SY5Y using 55214-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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GDI1 Polyclonal antibody

GDI1 Polyclonal Antibody for WB, ELISA
Cat No. 55214-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GDI 1, GDI1, GDIL, GDP dissociation inhibitor 1, MRX41, MRX48, Oligophrenin 2, OPHN2, Protein XAP 4, Rab GDI alpha, RABGD1A, RABGDIA, XAP 4, XAP4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 800 μg/ml
20 μL, 800 μg/ml
150 μL, 800 μg/ml

¥67,000/ 150 μL

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Tested Applications

Positive WB detected inSH-SY5Y cells, human brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55214-1-AP targets GDI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name GDP dissociation inhibitor 1
Calculated molecular weight 51 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberNM_001493
Gene Symbol GDI1
Gene ID (NCBI) 2664
RRIDAB_10858798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31150
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55kda protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034). Defects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41). Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48). The antibody is specific to GDI1. And it has no cross reaction to GDI2.

Protocols

Product Specific Protocols
WB protocol for GDI1 antibody 55214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols