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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GDI1 Monoclonal antibody, PBS Only

GDI1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E1G1

Cat no : 66434-1-PBS

Synonyms

GDI 1, GDI1, GDIL, GDP dissociation inhibitor 1, MRX41, MRX48, Oligophrenin 2, OPHN2, Protein XAP 4, Rab GDI alpha, RABGD1A, RABGDIA, XAP 4, XAP4

Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using GDI1 Monoclonal antibody (66434-1-Ig)

    WB analysis of U-251 using 66434-1-Ig (same clone as 66434-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using GDI1 Monoclonal antibody (66434-1-Ig)

    WB analysis of Neuro-2a using 66434-1-Ig (same clone as 66434-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using GDI1 Monoclonal antibody (66434-1-Ig)

    WB analysis of rat brain using 66434-1-Ig (same clone as 66434-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using GDI1 Monoclonal antibody (66434-1-Ig)

    WB analysis of mouse brain using 66434-1-Ig (same clone as 66434-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GDI1 Monoclonal antibody (66434-1-Ig)

    WB analysis of HEK-293 using 66434-1-Ig (same clone as 66434-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GDI1 Monoclonal antibody (66434-1-Ig)

    IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GDI1 Monoclonal antibody (66434-1-Ig)

    IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GDI1 Monoclonal antibody (66434-1-Ig)

    IF Staining of MCF-7 using 66434-1-Ig (same clone as 66434-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66434-1-Ig (GDI1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66434-1-PBS targets GDI1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GDI1 fusion protein Ag17511 相同性解析による交差性が予測される生物種
Full Name GDP dissociation inhibitor 1
Calculated molecular weight 51 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC000317
Gene symbol GDI1
Gene ID (NCBI) 2664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55 kDa protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034)