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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GDI2 Polyclonal antibody

GDI2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 55096-1-AP

Synonyms

GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human skin tissue using GDI2 Polyclonal antibody (55096-1-AP)

    IHC staining of human skin using 55096-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using GDI2 Polyclonal antibody (55096-1-AP)

    IHC staining of human skin using 55096-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GDI2 Polyclonal antibody (55096-1-AP)

    IHC staining of human liver using 55096-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GDI2 Polyclonal antibody (55096-1-AP)

    IHC staining of human liver using 55096-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive IHC detected inhuman skin tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55096-1-AP targets GDI2 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name GDP dissociation inhibitor 2
Calculated molecular weight 51 kDa
GenBank accession numberNM_001494
Gene symbol GDI2
Gene ID (NCBI) 2665
RRIDAB_11183032
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). The antibody is specific to GDI2. And it has no cross reaction to GDI1.

Protocols

Product Specific Protocols
IHC protocol for GDI2 antibody 55096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols