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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GDI2 Monoclonal antibody (60078-1-Ig)

    WB analysis using 60078-1-Ig (same clone as 60078-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60078-1-Ig (GDI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GDI2 Monoclonal antibody (60078-1-Ig)

    WB analysis of HepG2 using 60078-1-Ig (same clone as 60078-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60078-1-Ig (GDI2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GDI2 Monoclonal antibody (60078-1-Ig)

    IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GDI2 Monoclonal antibody (60078-1-Ig)

    IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GDI2 Monoclonal antibody (60078-1-Ig)

    IHC staining of human liver cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GDI2 Monoclonal antibody (60078-1-Ig)

    IHC staining of human liver cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GDI2 Monoclonal antibody (60078-1-Ig)

    IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GDI2 antibody (60078-1-Ig, Clone: 4B8F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GDI2 Monoclonal antibody (60078-1-Ig)

    IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 60078-1-Ig (GDI2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GDI2 Monoclonal antibody (60078-1-Ig)

    IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunofluorescent analysis of Hela cells, using GDI2 antibody 60078-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

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GDI2 Monoclonal antibody, PBS Only

GDI2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60078-1-PBS
Clone No.4B8F3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FLJ16452, FLJ37352, GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60078-1-PBS targets GDI2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GDI2 fusion protein Ag4613 相同性解析による交差性が予測される生物種
Full Name GDP dissociation inhibitor 2
Calculated molecular weight 47 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC005145
Gene Symbol GDI2
Gene ID (NCBI) 2665
RRIDAB_2232457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50395
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.