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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GDNF Polyclonal antibody

GDNF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26179-1-AP

Synonyms

ATF, ATF1, ATF2, GDNF, HFB1 GDNF, hGDNF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GDNF Polyclonal antibody (26179-1-AP)

    WB analysis using 26179-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26179-1-AP (GDNF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GDNF Polyclonal antibody (26179-1-AP)

    IHC staining of mouse brain using 26179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26179-1-AP (GDNF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GDNF Polyclonal antibody (26179-1-AP)

    IHC staining of mouse brain using 26179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26179-1-AP (GDNF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using human GDNF Polyclonal antibody (26179-1-AP)

    IF Staining of SH-SY5Y using 26179-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GDNF antibody (26179-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inDU 145 cells, HEK-293 cells, PC-3 cells, U-251 cells, U-87 MG cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26179-1-AP targets GDNF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GDNF fusion protein Ag24068 相同性解析による交差性が予測される生物種
Full Name glial cell derived neurotrophic factor
Calculated molecular weight 211 aa, 24 kDa
Observed molecular weight 32-40 kDa
GenBank accession numberBC069369
Gene symbol GDNF
Gene ID (NCBI) 2668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glial-derived neurotrophic factor (GDNF) is a member of the transforming growth factor (TGF)-α family and exhibits potent neuroprotective activity. GDNF is a potent promoter of neuronal survival in the CNS and peripheral nervous system (PNS). It has a relatively high speciicity for dopaminergic neurons and thus become an efective treatment for Parkinson's diseases (PD), with clinical trials currently in progress. Protein dimers of GDNF have a reported molecular weight of 32-40 kDa(PMID: 17982417, PMID: 23251488, PMID: 11429269).

Protocols

Product Specific Protocols
WB protocol for GDNF antibody 26179-1-APDownload protocol
IHC protocol for GDNF antibody 26179-1-APDownload protocol
IF protocol for GDNF antibody 26179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols