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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GDNF Recombinant antibody

GDNF Recombinant Antibody for IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230358A10

Cat no : 83087-1-RR

Synonyms

Glial cell line-derived neurotrophic factor, ATF2, ATF1, ATF, Astrocyte-derived trophic factor

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using GDNF Recombinant antibody (83087-1-RR)

    IHC staining of mouse brain using 83087-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83087-1-RR (GDNF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GDNF Recombinant antibody (83087-1-RR)

    IHC staining of mouse brain using 83087-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83087-1-RR (GDNF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GDNF Recombinant antibody (83087-1-RR)

    IHC staining of mouse brain using 83087-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83087-1-RR (GDNF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GDNF Recombinant antibody (83087-1-RR)

    IHC staining of mouse brain using 83087-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83087-1-RR (GDNF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GDNF Recombinant antibody (83087-1-RR)

    IF Staining of mouse brain using 83087-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GDNF antibody (83087-1-RR, Clone: 230358A10 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GDNF Recombinant antibody (83087-1-RR)

    IF Staining of mouse brain using 83087-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GDNF antibody (83087-1-RR, Clone: 230358A10 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using GDNF Recombinant antibody (83087-1-RR)

    IF Staining of SH-SY5Y using 83087-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GDNF antibody (83087-1-RR, Clone: 230358A10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of U-251 cells using GDNF Recombinant antibody (83087-1-RR)

    FC experiment of U-251 using 83087-1-RR

    (blue)1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human GDNF (83087-1-RR, Clone:230358A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using GDNF Recombinant antibody (83087-1-RR)

    FC experiment of SH-SY5Y using 83087-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Anti-Human GDNF (83087-1-RR, Clone:230358A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83087-1-RR

    Affinity and Kinetic Characterization of 83087-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83087-1-RR against Human GDNF were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells, U-251 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83087-1-RR targets GDNF in IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GDNF fusion protein Ag24068 相同性解析による交差性が予測される生物種
Full Name glial cell derived neurotrophic factor
Calculated molecular weight 211 aa, 24 kDa
Observed molecular weight 32-40 kDa
GenBank accession numberBC069369
Gene symbol GDNF
Gene ID (NCBI) 2668
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glial-derived neurotrophic factor (GDNF) is a member of the transforming growth factor (TGF)-α family and exhibits potent neuroprotective activity. GDNF is a potent promoter of neuronal survival in the CNS and peripheral nervous system (PNS). It has a relatively high speciicity for dopaminergic neurons and thus become an efective treatment for Parkinson's diseases (PD), with clinical trials currently in progress. Protein dimers of GDNF have a reported molecular weight of 32-40 kDa(PMID: 17982417, PMID: 23251488, PMID: 11429269).

Protocols

Product Specific Protocols
IHC protocol for GDNF antibody 83087-1-RRDownload protocol
IF protocol for GDNF antibody 83087-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols